mcherry mouse polyclonal antibody  (Cusabio)

Journal: Ecotoxicology and environmental safety

Article Title: Additive cardiotoxicity of a bisphenol mixture in zebrafish embryos: The involvement of calcium channel and pump.

doi: 10.1016/j.ecoenv.2023.115225

Figure Lengend Snippet: Fig. 4. Effects of BPs on cardiomyocyte development. Representative confocal microscopy image of TG(myl7:NLS-mCherry) ZFEs at 48 hpf exposed to 0.05% DMSO (A) or Mix 2 (BPA 8.7 µM + BPF 18 µM + BPAF 1.3 µM + BPB 4.2 µM), which induced approximately 40% heart rate reduction (B). BP exposure did not affect the cardiomyocyte count (C), based on the t test (n = 10). BP exposure, which induced approximately 30% heart rate reduction, did not affect mRNA expression of genes related to cardiomyocyte development: hand2 (D) and nkx20.5 (E). Only BPF and BPAF significantly downregulated bmp4 mRNA expression (F), based on the one- way ANOVA test followed by Dunnett’s post hoc analysis (n = 3). SC: DMSO 0.05%, BPA: 25 µM, BPF: 60 µM, BPAF: 4 µM, BPB: 12 µM, Mix 1: BPA 6.6 µM + BPF 13.6 µM + BPAF 1 µM + BPB 3.2 µM. ns, P ≥0.03; *, P ≤0.03; **, P ≤0.002; *** , P ≤0.0002; *** *, P ≤0.0001. The data are presented as means ± standard deviations.

Article Snippet: Embryos were stained with mCherry Mouse Polyclonal Antibody (Cusabio, Houston, TX, USA), Goat Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, and Alexa Fluor Plus 594 (Invitrogen) according to a previously described protocol (Hammond-Weinberger and ZeRuth, 2020).

Techniques: Confocal Microscopy, Expressing

Journal: Virus Research

Article Title: Nonstructural protein NS17 of grass carp reovirus Honghu strain promotes virus infection by mediating cell-cell fusion and apoptosis

doi: 10.1016/j.virusres.2023.199150

Figure Lengend Snippet: Subcellular localisation of NS17 in GCO cells. (A) Following transfection with pmCherry-N1-NS17 or pmCherry-N1 for 48 h, GCO cells were fixed and stained with DAPI (blue) and inspected by confocal microscopy. Blue fluorescence shows the nucleus, and red fluorescence shows the distribution mCherry-tagged protein. Scar bar = 20 μm. (B) Following transfection with pcDNA3.1-flag-NS17, pmCherry-N1-NS17 or empty vectors for 48 h, GCO cells were lysed and analyzed by western blotting.

Article Snippet: Finally, cells were collected to assess expression of pcDNA3.1-flag-NS17 and pmCherry-N1-NS17 by western blotting using mouse anti-mCherry-Tag polyclonal antibody (#AE002; ABclonal, Wuhan, China) and mouse anti-DDDDK-Tag antibody (#AE092; ABclonal).

Techniques: Transfection, Staining, Confocal Microscopy, Fluorescence, Western Blot